maxpar water (fluidigm)

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fluidigm maxpar water
Maxpar Water, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxpar+water/Maxpar+Water/pm41896367-365-15-17
Average 95 stars, based on 199 article reviews

maxpar water - by Bioz Stars, 2026-09

95/100 stars

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Staining:

Article Title: Nivolumab plus ipilimumab induce hyper-progression in renal medullary carcinoma: results of a phase II trial and preclinical evidence
Article Snippet: .. Immediately prior to data acquisition, samples were then washed with Maxpar Cell Staining Buffer and then with subsequent washes in Maxpar Water (Standard BioTools) to remove buffer salts. .. Next, the cells were resuspended at a concentration of 1 × 10 6 cells/mL in Maxpar Water containing a 1:10 dilution of EQ Four Element Calibration Beads (Standard BioTools) and filtered through a 35 μm nylon mesh filter cap (Corning, Falcon).

Article Title: Disruption of the Brain-Spleen Axis Impairs Monocyte-Microglia Communication and Accelerates Disease Progression in a Model of Amyloidosis
Article Snippet: Finally, the samples were washed twice and kept in 4% Formaldehyde (Pierce) with Iridium (125 nM) at 4°C overnight. .. On the day of analysis, samples were washed twice with MaxPar Cell Staining Buffer (Fluidigm), then twice with MaxPar water (Fluidigm), resuspended at a concentration of 300K cells/ml in 1:10 EQ Four Element Calibration Beads (Fluidigm) in MaxPar water, and filtered via a 35 um mesh before acquisition using a CyTOF 2 upgraded to Helios system (Fluidigm). ..

Cytometry:

Article Title: Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction.
Article Snippet: .. Thawed samples were washed with Maxpar Water (Standard BioTools), aliquoted and stored as pellets until acquisition on the CyTOF XT mass cytometer (Standard BioTools). .. Data clean-up and analysis was performed using CyTOF software (Standard BioTools) and the cloud-based software OMIQ (Dotmatics).

Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons.
Article Snippet: Compensation beads (OneComp eBeads Compensation Beads, Invitrogen, #01-111-42) stained with 1 μl of each antibody were also prepared. .. The following day, cells and compensation beads were washed twice with CSB and twice with Maxpar water (Fluidigm), mixed with a 1:10 volume of EQ Four Element Calibration Beads (Fluidigm) before acquisition on a Helios Mass Cytometer (Fludigm) using the HT injector. qRT-PCR Freshly isolated PBMCs were stimulated with 250 U/ml of IFN-α1 or IFN-β in R10 or left unstimulated (3 × 106 cells/sample) in a volume of 2 ml in a non-tissue culture treated 12-well plate and incubated for the time indicated in the figure legend at 37 °C. .. RNA was extracted using a RNeasy Mini Plus Kit including a gDNA eliminator column step, according to the manufacturer’s instructions. cDNA synthesis was performed with SuperScript IV reverse transcriptase (Thermo Fisher Scientific) and oligo(dT)12-18 primers (Thermo Fisher Scientific).

Article Title: Automated GMP-compatible production of universal CAR Tregs for organ-targeted tolerance induction
Article Snippet: For cell identification, samples were labeled with DNA intercalators ( 191 Ir and 193 Ir, Standard BioTools), resuspended in Maxpar Fix and Perm Buffer (Standard BioTools) with 4% methanol-free paraformaldehyde (PFA, ThermoFisher Scientific), and incubated overnight at 4 °C.The cells were cryopreserved in FBS containing 10% (v/v) DMSO and stored at −80 °C until analysis. .. Thawed samples were washed with Maxpar Water (Standard BioTools), aliquoted and stored as pellets until acquisition on the CyTOF XT mass cytometer (Standard BioTools). .. Thawed samples were washed with Maxpar Water (Standard BioTools), aliquoted and stored as pellets until acquisition on the CyTOF XT mass cytometer (Standard BioTools).

Quantitative RT-PCR:

Isolation:

Incubation:

Concentration Assay:

Gentle:

Article Title: Cytometric Atlas of Combination Immunotherapy in Pancreatic Cancer: Blood-Based Signatures Reveal Vaccine and Checkpoint Inhibitor Responses
Article Snippet: .. After washing in Maxpar water for 5 minutes (Standard Biotools), the slides were placed in a pre-heated target retrieval solution (Antigen Retrieval Agent (DAKO), pH 9, 1:10 in Maxpar water) at approximately 90–95°C for one hour, followed by a 10-minute wash in Maxpar PBS with gentle shaking. ..

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